Summary
Type: SPE
Application #: 300320
Catalog#: 730013 - SPE Cartridges, Chromabond, C18 ec, 3ml, PP, 500mg, 50/pk
Detection:
Conditions: Conditioning of the C18 ec column:
1 column volume methanol, then 1 column volume 0.1 N hydrochloric acid
Sample application:
Slowly force or aspirate 1 ml sample through the column
Column washing:
2 x 1 ml 0.1 N hydrochloric acid; dry column under vacuum for 30 sec; aspirate
1 ml n-hexane through the column; dry column under vacuum for 1 min
Conditioning of the OH column:
1 column volume dichloromethane, then pour 2 ml dichloromethane into the
column, do not apply vacuum
Sample transfer:
Place C18 ec column onto the OH column. Pour 2 x 1 ml dichloromethane through
the columns; let each portion of dichloromethane remain in the C18 ec packing
for 1 min, then aspirate solvent through the columns. Remove C18 ec column.
Wash OH column with 1 ml dichloromethane and dry 1 min under vacuum.
Elution:
Leave 250 μl methanol in the column packing for 1 min, then elute benzoic acid
with 2 x 500 μl methanol
Authors: Macherey-Nagel
Source: LC department